Sentence examples for present in the vector from inspiring English sources

Hybridization was carried out using the RGA2 intron present in the vector pOle18-MIPS-006, which was labeled with α-32P dCTP radioisotope (BARC, India), using Decalabel DNA labeling kit (Fermentas) according to the manufacturer's instructions.

These initiation codons are in frame with the ATG initiation codon present in the vector (within the NcoI restriction site, see Figure 1B).

PvuI was used for linearization of the plasmid as only one restriction site is present in the vector and which is located in the ampicillin resistance gene.

pSP72-B3-tk-B5::B1-Kozak-NLS-LacZ-Stop-B2 had a very low level of activity in both cell lines in keeping with the idea that no exogenous enhancer was present in the vector backbone.

However, because vectors are composed of retroviral portions including the LTR sequence that can activate additional viral sequences present in the vector, the development of alternative methods has been encouraged [2], [3], [20].

These data demonstrate that an intact Ter sequence is necessary and sufficient for optimal binding to the Tus protein, allowing the effective binding of Tus-fusion proteins to Ter site(s) present in the vector and supporting the application of the Tus-Ter system for protein microarray fabrication.

As expected there was no FABP1 present in the vector-transfected group while the FABP1 cDNA transfected showed presence of the protein.

It thus appears that when a high expression of the IRES-controlled cistron is needed, one should verify the integrity of the EMCV IRES present in the expression vector used, and shift to a vector containing the wild type form of the EMCV IRES.

Because lentiviral vectors are produced by triple transient transfection of 293T cells, plasmid DNA is present in the concentrated vector requiring a DNase treatment of each lentiviral vector sample prior to RT-qPCR (results not shown).

The hard and soft limits for each node are respectively presented in the vectors H d and S d (index "d" stands for diagonal).

The early version of this work [20] produced an interpretation based on the impact of combinations of fragments present in the feature vector of a query structure.