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GS and SMAA were both present in stratum granulosum and inner parts of epidermis (Figure 1F).
Remarkably, GS (at a moderate level) and β-catenin were both present in stratum basale, the actual proliferative compartment, and in stratum spinosum.
RNA from tape-stripped skin identified genes present in stratum corneum that are differentially expressed between melanoma, naevus and normal skin specimens.
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It was present in the stratum basale, stratum spinosum, and stratum granulosum with the vast majority of positive cells being in the supra-basal layers.
However, the NMDA receptor subunit NR2D is only present in the stratum oriens of the CA1 and CA2, the stratum lucidum of CA3 and the inner third of the molecular layer of the dentate gyrus [53].
To determine the minimum number of DNases present in the stratum corneum, we separated a protein extract from human stratum corneum by anion exchange chromatography and tested DNase activities of the fractions.
Alternatively, the RNA from malignant melanocytes could be sampled by EGIR if the malignant cell is present in the stratum corneum or if tape stripping extends well below into the stratum granulosum.
Hopf et al. (2014) assumed that DEHP first permeates the stratum corneum and is then hydrolyzed, arguing that cutaneous esterases are generally not present in the stratum corneum.
Importantly, the fact that the DNase is present in the stratum corneum of plantar epidermis, which lacks sebaceous glands, demonstrates that sebocytes are not essential for the formation of stratum corneum DNase activity.
Strata radiatum and oriens exhibited moderate expression while the lowest densities of SERT-IR fibres are present in the stratum pyramidale (S. Py).
In most cases HIF areas were only present in the stratum corneum, while LIF areas were also present in the viable epidermis.
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