Approximately 1 ng of human template (genomic) DNA was present in the multiplex PCR amplifications.
The detection sensitivity for each virus was 20 200 copies per reaction when the assay was performed separately for each virus, and the sensitivity was 1000 copies per reaction when all of the 16 pre-mixed viral targets were present in the multiplex assay.
Recently, further support for an involvement of the ASMT gene in ASD was presented in a Multiplex Ligation-dependent Probe Amplification (MLPA) study showing that a duplication in the ASMT gene was significantly more common in ASD, as compared to controls [ 20], suggesting that the expression of the ASMT protein may be altered in ASD patients.
Furthermore, the HotStarTaq polymerase present in the QuantiTect Multiplex PCR buffer in the PCR master mix is inactive at 30°C.
In the present study, multiplex PCR and SYBR Green real-time PCR were developed to facilitate the simultaneous detection of three important rice pathogens, Xanthomonas oryzae pv.
This work presents an approach for link prediction in Multiplex networks where the associations are learned from the multiple layers of networks for link prediction purposes.
Results showed that the LNA/2′OMe-FISH method performed well in multiplex experiments and presented a good correlation with total and cultivability counts, regardless of the cells physiological state.
We employed barcodes of varying length in EXPRSS, with 8 fold multiplexing for experiments presented in this manuscript and 16 fold multiplexing at present in our lab.
In such future studies, it will also be possible to evaluate further the eventual diagnostic value of the individual peptides included in the present multiplex assay.
In this study, we present a multiplex real-time PCR that is a robust, reliable and rapid method for the detection of the most prevalent carbapenemases blaOXA-48, blaVIM, blaIMP, blaNDM and blaKPC, and is suitable for screening of broth cultured rectal swabs and for identification of carbapenemase genes in cultures.
