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At day 13, all (colored) feces pellets present in each petri dish were collected using a sterile 10 μl pipette tip.
Samples of field water and ultrafiltered field water (100 µl each) were spread on the solidified media present in petri dish.
The percentage of AP-positive cells in a given 60-mm dish was estimated based on the total cell count at D20 of induced differentiation, the number of PGC-like cells estimated to be present in a dish based on morphological assessment, and the number of PGC-like cells that stained positive for AP.
Tumor blood burden is reported as the total number of cancer cell colonies present in a dish normalized to the volume of blood plated.
The number of myofibrils present within myotubes was counted in fifty randomly chosen microscope fields (3 culture dishes, 50 fields in each dish) at a magnification of x 400.
A dish of fresh water was always present in each rat's cage.
The mooli, or white radish, in Luu's recipe is a puzzle, though – it doesn't appear to be present in the finished dish, but I'm not sure what part it might play in flavouring the stock, given that it doesn't have much flavour of its own.
Five days after plating, less than 1.5×106 cells were present in the culture dish (250,000 cells were plated) compared with more than 2×106 in the untreated group.
Formaldehyde (36.5%; Sigma) was added to the dishes to final concentration of 1% in each dish.
Wounds were marked and scored in each dish.
Chili heat is often present in Sichuan dishes, but not always.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com