Importantly, the HA-tagged DAT that is present in DAT-KO/HAD-Tg mice is functional, as it is able to partially rescue the DAT-KO hyperactive phenotype.
Although in the present study DAT is more sensitive than ELISA, the subjective nature and longer waiting time before reading the results might limit widespread and large scale usage of DAT for epidemiological studies [ 61].
Results in (% of total amount present at 1095 DAT).
Results are presented as % of the total amount still being present in the soil at 1095 DAT.
Overall, this literature is consistent with the present results which indicate reduced fronto-cortical activity in DAT KO mice, indicating that many DAT KO phenotypes are not simply the result of increased DA function, but of the consequent changes in activity within the overall circuitry of which the dopaminergic projections are just one part.
Our results do not support the hypothesis that large differences in striatal DAT availability are present in smokers compared to ex-smokers and healthy volunteers with no history of smoking.
In contrast, sparse DAT immunolabeling was present in the rat dorsal thalamus; it was mainly located in the mediodorsal, paraventricular, ventral medial, and ventral lateral nuclei.
The DAT-ir axons present in the parasagittal monkey and rat sections were drawn using a Neurolucida set-up (MicroBrightField, Colchester, VT) attached to another Zeiss Axioskop microscope as described earlier (García-Cabezas et al. 2007).
As a matter of fact, DAT is not only present in striatal synapses: DA neurons in ventral mesencephalon, i.e. in the Substantia Nigra (SN) pars compacta (SNc; cell group A9) and in the Ventral Tegmental Area (VTA; cell group A10), are enriched with moderate to high DAT immuno-reactive intensity [ 78, 79], where it is concentrated in perikarya, dendrites, and axons.
In the present study, the DAT and IBZM SPECTs were done on a hybrid gamma-camera system, an Infinia Hawkeye (General Electric, Milwaukee, WI, USA) at baseline and at sequential timepoints up to 3 years from inclusion.
I doan' take no stock in dat...
