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In a bioreactor culture of genetically engineered Pichia pastoris secreting a bivalent immunotoxin, 64% of the secreted immunotoxin was present in aggregate forms and this resulted in a loss of bioactivity.
Other abundant RNAs, such as mRNAs, snRNAs, and snoRNAs, are present in aggregate at levels that are about 1-2 ofders of magnitude lower than rRNA and tRNA.
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In soils and aquifers, clay minerals and other reactive surfaces are often present in aggregates.
Scanning electron microscopy showed that synthesized nanoparticles exhibited spherical nanospheres with a size of 150 200 nm showing selenium-nanospheres were present freely around the cells and also present in aggregates connected to bacterial cell mass [40].
The results showed that the percentage of P. aeruginosa FRD1 but not S. aureus RN6390 in the cultures that were present in aggregates were decreased by A101 (data not shown).
We quantified the number of intracellular bacteria residing individually in phagosomes, the cytoplasm, autophagic vacuoles, or lysosomes, or being present in aggregates or acidic aggregates.
It is currently unknown whether UBQLN2 is present in aggregates in other neurodegenerative diseases and a first study did not detect mutations in UBQLN2 in FTD [ 79].
This result indicates that pgl-1 mutants cultured at 25° are defective in timely repair of meiotic DSBs, most likely due to the fact that SYP-1 is present in aggregates rather than on chromosomes.
Thus, a second hypothesis is that, in the supersaturated solution of m-ABA in DMSO at the start of the crystallization experiment, there is an increase in the proportion of non-zwitterionic m-ABA molecules present in aggregates containing intermolecular hydrogen bonds with the NH2 group as the acceptor.
The finding that the SOD1 protein was actually present in aggregates in fALS postmortem patients and also transgenic mice suggested that, similar to other neurodegenerative diseases, the aggregating pathology of ALS could be linked to mutations in this gene (Bruijn et al., 1998).
Because proper assembly of the SC is necessary to complete DSB repair by crossover formation (Colaiacovo et al. 2003), we tested whether meiotic DSB repair might be defective in the pgl-1 mutant when SYP-1 is present in aggregates rather than assembled into SC.
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