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Sodium interacts with the oxygen present at the underlined position.
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A preference for position -620 (nt A in sequence ccAtccc) is consistent to the view that the Initiator (Inr) has the consensus element YYAN(T/A YY (the underlined position indicates the TSS) [ 18].
The true positives were established by confirmation with the list of the underlined positions on Table 2.
The underlined positions from Table 2 that were not identified as key positions were classified as false negatives.
In these sequences, glutamate is present at the 253rd position.
The location of the DNA mismatch is noted at the underlined base.
For example, seco-CI-InBf (5a) and seco-CI-TMI (5c) demonstrated specificity for AT-rich sequences, particularly by reacting with the underlined adenine-N3 position of 5′-AAAAA 865 -3′.
In addition, the numbers differed among positions (GH = 14.7, 9 df, P = 0.0995), with a few underlined positions showing low or significant P values (for positions –5 and –3, P = 0.078; for position –2, P = 0.007).
The residues in italics and underlined, indicate the amino acids that are not presented in the specific position from these known neutralized discontinuous motifs (see Figure 1(c)).
The sequence context for the oligonucleotide design was the third position of the N- ras codon 61 (CA A, where the underlined A is the position of the adduct).
In this case, the sequence context was the human N- ras codon 12 (G GT, where the underlined G indicates the position of the adduct).
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