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2-Naphtol had no significant influence if present at the same concentration level as 1-naphtol analyte; however, its higher concentration resulted in a rise of the band at 490 nm in the DR-UV spectra and overestimated 1-naphtol analytical value as a result.
The use of a range of target concentrations to collect observations was very important in establishing the general applicability of this model: not only did it point to a weakness in the free energy model but it reflects the experimental reality that not all genes or genomic segments are present at the same concentration in most microarray experiments.
The graph models the scenario that cells contain an additional phosphatase PPX, present at the same concentration as PP2A-B55 (250 nM), which targets pEndos.
For simplicity, multiple transcripts are present at the same concentration in this modified design, rather than each transcript at a different concentration.
For measurements of Δψm, cells were loaded with 25 nM tetramethylrhodamine methylester for 30 min at room temperature and the dye was present at the same concentration in all solutions throughout the experiment.
Although in principle the model can represent cells where proteins are present at different concentrations and interact with different affinities, here we focus on the topological properties of the network and we use a simple model where proteins are all present at the same concentration and interact with the same "strength".
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** Components present at the same concentrations in all mixes include 200 μM dNTPs, Taq DNA pol.
This showed that in budded G2 cells, all cohesin subunits were present at approximately the same concentration.
The uppermost band is present at approximately the same concentration in both exposed and unexposed samples and was identified as lysozyme.
Positive control wells consisted of the individual microorganism under investigation inoculated in 100 μL nutrient broth while negative control wells contained DMSO at the same concentration present in the test wells.
Control cells were treated with DMSO alone at the same concentration present in the test substance being evaluated.
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