Sentence examples for present at the active site from inspiring English sources
Proteases are also subdivided based on the nature of the amino acids or other substituents present at the active site, which participate in the bioctalytic mechanism into serine, aspartic, cysteine, metallo-, glutandc, and threonine proteases.
Using site-directed mutagenesis, we exchanged the amino acids present at the active site of mAOX1 by the ones found in XOR.
When PMP is present at the active site, the loop is open, thus allowing substrate entry and product release.
Another possibility is to form a covalent bond between nucleophilic residues present at the active site and analogs of the intermediary enolate (Yamamoto et al. 2007).
ADP and one magnesium ion are present at the active site in both molecules in the ASU, and there is strong electron density to validate their presence.
Interestingly, the three-dimensional structures of the PV, HRV, HAV, FMDV, and CV 3C proteases indicate that a third amino acid, either aspartate or glutamate, is present at the active site, as in all serine proteases.
The sequence data also suggested that there are thiol groups presented at the active site that are involved in binding or catalysis, or that such groups are essential for maintaining a proper tertiary structure of the enzyme [ 34- 36].
A sequence analysis of AChEs from 68 species and three-dimensional models of the greenbug and English grain aphid AChEs reported herein reveal that a cysteine residue is present at the active sites of greenbug and aphid AChEs but absent at those of mammalian AChEs.
In agreement with structures of glutathione S-transferases from the theta and phi classes, a serine residue (Ser17) is present in the active site, at a position that would allow it to stabilise the thiolate anion of glutathione.
Unexpectedly, endogenous coenzymeA was present in the active site.
At the end of the reaction, but before product release, the nucleophilic water is used up and hence should not be present in the active site.
