These two insertions are in phase and are present at the frequency of 0.3342%.
Being an aged population, the diseases or conditions involved are not only specific to older persons, but are also present at a frequency that is higher than expected, e.g., heart failure, arrhythmia, ischaemic heart disease, anxiety/depression, obesity, thyroid disease, asthma, osteoporosis, generalised artherosclerosis and chronic liver disease.
Overall EEG revealed the expected peak of alpha in the dataset ranging from 7.5 to 12.25 Hz among the different subjects (see supplementary Table S1 for more details) and another peak is present at a frequency range that corresponds with gamma (40 80 Hz).
However, these SNPs are present at moderate frequency suggesting that they may have limited impact on the evolution of miRNA function.
Dinucleotides that are under-represented in coding regions thus appear as codons that are present at low frequency.
We chose to identify blocks for which at least 97% of individuals have a haplotype allele that is present at a frequency of at least 2.8%.
Moreover, we identified 5 mutations present at low frequency (below 5%) that previously failed detection with Sanger sequencing.
Whether these arise during reprogramming or represent changes that were present at low frequency in the parental cells and whether they are selected against during iPSC culture is unknown.
Custom Perl scripts were used to exclude SNPs supported by fewer than 5 sequence reads or that were present at a frequency of less than 1% of informative reads.
Thus, when sampling a population of random mutants, each sequenced plasmid provides information on about seven different barcodes and sequencing 20 to 30 plasmids can provide a sufficiently large sample size to identify barcodes that are present at high frequency.
