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Because it is possible that the position of the GFP and/or RFP molecules are affected by assay conditions or the presence of test compounds, addition controls are needed.
The proportion of works with and without sampling problems was evaluated considering the journal impact factor, period of publication, community status (urban x rural), sample type, presence of testing hypothesis and presence of research questions.
The reduction of FeCl3 in the presence of test sample was measure of reducing ability.
Biofilms on the surface of polystyrene plates were grown in the presence of test compounds as detailed above.
Crystal violet assay was used for the detection of the biofilm inhibition in the presence of test compounds.
Fig. 5 Percent inhibition of the biofilm formation by S. mutans in the presence of test compounds.
Therefore, additional experiments for differentiating these kinds of mechanism, ergosterol quantitation and ergosterol binding assays were performed for Candida cells in the presence of test compounds.
These processes are also likely influenced by the presence of test organisms [11], e.g., through release of organic substances such as algal exudates.
The treated and untreated cells were allowed to grow in the presence of test compounds by further incubating the plates for 24 h.
Finally, the presence of test files also influences bug localization results, since bug reports related to these files are very likely to be localized (Section 3.4.3).
E value expresses the effect of the test sample or the positive control on the enzyme activity articulated as the percentage of the remaining activity in the presence of test sample or positive control.
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