Sentence examples for prerequisite reference from inspiring English sources
With the advantage of no prerequisite reference genome sequence requirement, cDNA-AFLP has been used to understand regulation mechanisms under various biotic and abiotic stress conditions that influence patterns of global gene expression in diverse crop species [ 20- 22].
As a prerequisite, robust reference analysis methods for carbohydrates are needed.
A set of marker genes that are regulated specifically by growth hormones and environmental factors is a prerequisite for determining reference gene expression stability under various experimental conditions.
RNA-seq technology, unlike microarray, does not depend on the prerequisite knowledge of the reference transcriptome [ 24].
Moreover, a mean expression level lower than the maximum expression level subtracted with 2 SD is a prerequisite for a candidate reference gene.
Being constitutively expressed however does not necessarily meet the prerequisites for a good reference gene, which also needs to display a sufficiently small variation in expression among different tissues.
The statistical prerequisites for a valid reference interval includes 1) that the sampled population is representative for the population from which the laboratory will receive samples, 2) that the number of samples is large enough, and 3) that the statistical methods used for calculation of the reference interval are valid.
Although a majority of higher plant genomes have been sequenced by whole genome shotgun strategy [ 4], hierarchical approach of genome sequencing with the intermediate in form of physical contig map anchored to individual chromosomes is a prerequisite to obtain high-quality reference sequences [ 5, 6].
Acknowledging the parents' frames of references is a prerequisite for successful management.
The latter method is computation-and-time efficient but relies on the availability of an accurate reference with strong prerequisites such as comprehensive genome coverage and proven representativeness.
Thus, appropriate reference genes are a prerequisite for qRT PCR (Dankai et al. 2015).
