Sentence examples for preparing the template from inspiring English sources

The second method utilizes fluoren-9-ylmethyl N- 11-mercaptoundecyl) carbamate (FMUCe) iN- 11-mercaptoundecyluN- 11-mercaptoundecylring the template.

After preparing the template wafers, polydimethylsiloxane (PDMS) (Sylgard 184, Dow/Corning, Midland, MI) was poured across the wafers, and then cured at 70 °C for one hour.

The resulting construct was used as a template DNA for the second PCR using T7 promoter and terminator primers (P22 and P23) to prepare the template DNA for GFP11 synthesis in the PURE system (Supplementary Fig. 15).

Fawzia Fahim: prepared the template polymer used in the study.

To prepare the template for PCR amplification of the target regions for in vitro transcription (IVT), total RNA was prepared from 200 liquid N2 freeze-dried whole w1118 flies using TRIzol® reagent (Invitrogen).

To prepare the template for pyrosequencing, biotinylated PCR products in a 10 µL aliquot were captured in 2 µL streptavidin-sepharose beads (Amersham Biosciences, Pisctaway, NJ) mixed with 40 µL binding buffer (10 mM Tris-HCl, 2 M NaCl, 1 mM EDTA, and 0.1% Tween-20, pH 7.6) and 35 µL doubly distilled water.

Genomic RCA product (prepared using the Operon Repli-G Kit) was used to prepare the template for shotgun sequencing.

Then we prepared the template and RT-PCR mix and added DNA to the mix with aerosol-resistant tips in two different rooms.

Two plasmids containing the cDNA sequences for the soluble or membrane-anchored encoding transcripts of hIL-5Rα, kindly provided by Prof. Dr. Jan Tavernier, were used to prepare the template for the standards.

In this work, therefore, the aging time of 4 h was employed to prepare the templates, which possess almost the same pore size and structure regardless of the TiO2 content.

The primers used to prepare the templates for An.