Sentence examples for preparing the sequencing from inspiring English sources

The products were purified using RNeasy Micro kit (Cat 74004, QIAGEN) and enriched with PCR for preparing the sequencing library.

The products were purified for the section of approximate 200 bp long using Qiaquick Gel Extraction Kit (Qiagen) and enriched with PCR for preparing the sequencing library.

The slight deviation from the expected 10% per sample is likely due to small pipetting errors when preparing the sequencing pool.

We are grateful to Dr. R.C. Venu at The Ohio State University (OSU) for preparing the sequencing library and the OSU Molecular Cellular Imaging Center (MCIC) for performing Illumina sequencing.

We note, that this is not due to the lack of a polyA tail on the histone H1.0 mRNA when preparing the sequencing libraries, since the synthesis of histone H1.0 is not cell cycle-regulated and its mRNA is polyadenylated [ 36, 37].

Therefore it is feasible to spend one hour or so preparing the sequence of configurations that will be used for testing a specific board.

GH prepared the sequencing samples, participated in the bioinformatic analyses, conducted the genotyping, and evaluated the genotyping data.

PB performed data analysis, LJ prepared the sequencing libraries and MK helped with the annotation of sequences obtained through deep sequencing.

These bacteria most likely grew in the samples during the prolonged storage before extraction of the nucleic acids used to prepare the sequencing libraries.

The Illumina mRNA-Seq Sample Prep Kit (Illumina, San Diego, CA USA) was used to prepare the sequencing library with 3 μg of nuclear RNA.

The ABI PRISM BigDye cycle-sequencing kit (Perkin Elmer, Foster City, CA), and specific primers, or the primers for the M13 forward and reverse priming sites of the pCR2.1 vector, were used to prepare the sequencing reactions.