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Therefore it is feasible to spend one hour or so preparing the sequence of configurations that will be used for testing a specific board.
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The products were purified using RNeasy Micro kit (Cat 74004, QIAGEN) and enriched with PCR for preparing the sequencing library.
We are grateful to Dr. R.C. Venu at The Ohio State University (OSU) for preparing the sequencing library and the OSU Molecular Cellular Imaging Center (MCIC) for performing Illumina sequencing.
The slight deviation from the expected 10% per sample is likely due to small pipetting errors when preparing the sequencing pool.
The products were purified for the section of approximate 200 bp long using Qiaquick Gel Extraction Kit (Qiagen) and enriched with PCR for preparing the sequencing library.
To prepare the sequence template (nucleotides 75-638 of 5'NTR of HAV), PCR products were treated with ExoSAP-ITR (Affymetrix, Inc., Santa Clara, CA), and then sequenced using a BigDye(R) Terminator v3.1 Cycle Sequencing Kit (Life Technologies, Tokyo, Japan).
GM conducted laboratory work and prepared the sequence alignments.
The Pregap4 modules were used to prepare the sequence data for assembly (quality analysis).
The trial biostatistician will prepare the sequence of treatments randomly permuted in blocks of unequal size.
SM carried out the molecular genetic studies and prepared the sequence alignment.
CLC and CLW prepared the sequence data and submitted it to GenBank.
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