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We thank Amparo Ruiz-Prado for preparing plant materials and Dr Zhonghua Chen for helpful discussion during the preparation of the paper.
A variety of methodologies exist for preparing plant organs for anatomical studies, many of which are sample dependent (Johansen, 1940; Sass, 1958; Feder and O'Brien, 1968; Carlquist, 1982; Ruzin, 1999; Keating, 2014).
Given that micropropagation is an economical and effective method to produce a large number of plant seedlings (Smith, 2000), development of micropropagation systems for candidate phytoextractors should be a good option to reduce the expense of preparing plant material.
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In Arabidopsis, the anthocyanin pathway is regulated in a circadian fashion, with flavonoid genes peaking at the end of the dark cycle, likely preparing plants for daybreak [ 21].
During the 1950s Daniel Arnon and other American biochemists prepared plant cell fragments in which not only the Hill reaction but also the synthesis of the energy-storage compound ATP occurred.
Local people are paid to prepare, plant, and care for seedlings in degraded areas.
In order to prepare plant transformation binary plasmids, pEnRD29A-PAP1 and pEnRD29A-CBF3 werecombinedned using LR clonase (Invitrogen, CA) with pFAJGW and pMDC99, respectively.
OB prepared plant materials.
HY prepared plant materials.
LL, XL, and ZG prepared plant materials.
CD prepared plant materials and carried out qPCR analysis ([email protected]).
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CEO of Professional Science Editing for Scientists @ prosciediting.com