Immediately after incubation, aliquots of the medium were removed for assay of insulin and glucagon [48], [49], [50] and the islets were prepared for measurement of NOS activities as described below.
Animals were grown for 96 hr in these conditions and then prepared for measurement on the COPAS BIOSORT (Union Biometrica).
Patellae were then further prepared for measurement of SO42- incorporation in the articular cartilage as previously described [ 26].
A typical fluorescence intensity histogram from 10 000 cells is shown in Figure 1 A. The cell culture had been exposed to a 10 nM concentration of quantum dots for 1 h, following which the excess nanoparticles were removed with a series of washes; after a further 24 h incubation period, cells were fixed and prepared for measurement (see Methods section).
Nanofluids prepared for measurements with this method were stable for several hours.
After the sample was prepared for measurements, the origin of the wafer coordinates was put relatively.
The starting mixtures of the MWCNTs' different fractions (0, 0.5, 1.0, and 2.5 %) were taken, so four types of the samples were prepared for measurements.
Shortly, subconfluent monolayer cell cultures (∼60% confluence) attached to polystyrene, FN or Matrigel were irradiated with 2 or 6 Gy, detached with trypsin/EDTA at defined time points (0, 12, 24, 36, 48 h) and prepared for measurements.
Non treated cells were also prepared for SERS measurement.
Samples without the AlN cap layer were also prepared for morphology measurement.
Rats were anesthetized with pentobarbitone (60 mgkg-1) and prepared for physiological measurement.
