Exact(7)
The process of preparing extracts for pharmaceutical industry from natural resources is a challenge to obtain effective constituents besides using resources efficiently considering public health and environment.
However, as an active element of knowledge Fleck indicates also "the mere use of alcohol in preparing extracts", adding however that "the actual usefulness of such extracts is a passive one and therefore a necessary consequence".
A Mini Lyophilizer (LTE Scientific Ltd ,UK) and rotary flash evaporator were used for preparing extracts.
All cells were treated with the cross-linker, DSP, before harvesting and preparing extracts as described in 'Materials and methods'.
The same cell cultures were also evaluated by preparing extracts in SDS and resolving aliquots on a Tris-Glycine or NuPAGE gel.
To increase the probability of capturing transient interactions, we pre-treated cells with the cross-linking agent dithiobis(succunimidylpropionate) (DSP) before preparing extracts and immune-precipitating Sgo1-TAP.
Similar(53)
To prepare extracts, adherent cells were trypsinized, centrifuged, and washed in PBS.
To compare the anticancer property of RGE, we prepared extracts of additional seven vegetables and 14 fruits in the way similar to that of RGE preparation.
We prepared extracts from single eggs and from single embryos in the earliest stages of development.
The Karginov et al. study employed similar growth conditions, and also prepared extracts 48 hours after transfections.
We generally prepare extracts from the yeast "noodles" in batches of six.
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