MICs for imipenem and rifampicin were determined by preparing doubling dilutions ranging from 0-64 μg ml-1 in a total volume of 50 μl NB.
If your pans are slightly larger, prepare double the ganache filling.
When making this pie, I like to prepare it as a single crust pie, but you may also prepare double the recipe and make a lattice top.
Total RNA was used to prepare double strand cDNA.
We also prepared double mutants for each of FABP5 (R109and129A) and FABP7 (R107asR127A), as binding pocket-destructive mutants [negative controls for 1-anilinonaphthalene-8-sulfonic acid (ANS) titration assay].
We prepared double dilutions of the drugs; the concentrations of the drugs in the Mueller Hinton broth ranged from 0.06 to 128 μg/mL (for antimicrobial agents) or 1.25 to 1280 μg/mL (for ascorbic acid derivatives).
To prepare double stranded 5'-labeled DNA, labeled NT or T strands were mixed 1 1 with unlabeled T or NT strands, respectively, and annealed in 10 mM Tris-HCl pH8.0, 50M M NaCl, 1 mM EDTA.
Thus, we prepared double-strand oligonucleotides with hemi-methylated and full-methylated CpG sites.
Stones were first vacuum impregnated and then mounted on borate silicate glass slides with clear low-viscosity cathodoluminescence-resistant epoxy impregnation to prepare double-sided polished, 20 µm-thick petrographic thin sections.
Especially, the dip-coating process is suitable to prepare double-sided coatings at the same time.
