Sentence examples for preparing competent from inspiring English sources

They also require a step of preparing competent cells which is a time consuming process [6, 7].

Saccharomyces cerevisiae FY834 (MATα; his∆200; ura3-52; leu2∆1; lys2∆202) was refreshed on YPD agar medium (per liter: 20 g glucose, 20 g peptone, and 10 g yeast extract) at 28 °C for 48 h and then used for preparing competent cells as described by Frozen-EZ Yeast Transformation II Kit (T2001, Zymo research, USA).

The nursing faculty shortage challenges us to prepare competent students.

Briefly, strain grown in LB to an optical density of 0.3 0.4 at 600 nm was used to prepare competent cells.

The correct plasmid was transformed into strain SW106 to prepare competent cells after induction of the λ recombination genes.

To prepare competent cells, L. pneumophila grown on BCYE agar plates were resuspended in 200 ml of sterile water to an OD600 between 0,5 1.

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Issues exist of funding, coordinating and managing CBE to prepare competent providers.

Chemically prepared competent cells are more widely used and work well in most cases for penetration of the plasmids.

The recombinant pHSG415 plasmids (pHSG415 containing locked or unlocked alleles) were first transformed into chemically prepared competent E. coli DHα cells, and then purified and transformed via electroporation (Bio-Rad) into LT2 strains.

To prepare competent cells, a 50 ml culture of JWCB005 was grown in LOD minimal medium at 75°C for 18 hours (to mid exponential phase) and 25 ml of the culture was used to inoculate a 500 ml culture of LOD (low osmolarity defined growth medium) supplemented with 40 μM uracil and a mixture of 19 amino acids (5% inoculum, v/v) [ 29].