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"The Complete Indian Housekeeper and Cook" does not perfectly map on to my experience — it is written for women who must manage "the help" (if you are someone who hires babysitters or house-cleaners, you'll find it interesting), and much of the practical advice — keeping a pantry, preparing Brains au Gratin — is outmoded, too.
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Ferrets and Wistar rats were used acutely for the purpose of obtaining and preparing brain slices.
Wistar rats were used acutely for the purpose of obtaining and preparing brain slices.
The procedure for preparing brain sections was performed as described previously [ 29].
To ascertain the long-term effects of Cdc42 deletion in vivo, we prepared brains from p120 Cdc42 f/f : Camk2a-Cre mice and conducted Goli-Cox staining.
To prepare brain lysates mice were euthanized using carbon dioxide followed by perfusion with PBS.
To prepare brain samples for immunostaining, Nissl and Fluoro-Jade B staining, and TUNEL labeling, mice were euthanized with carbon dioxide and perfused with 4% paraformaldehyde in phosphate-buffered saline (PBS).
To prepare brain cells, mouse brains were washed in PBS and minced in dissociation buffer.
We prepared brain microsomes from mouse neocortical tissue as described previously for the rat (Wong et al. 1997a).
The middle third of brains was sliced using a brain matrix (Braintree Scientific, Braintree, MA, U.S.A). to prepare brain slices for immunohistochemical and biochemical analyses.
To collect liver and brain, mice were perfused with PBS (pH 7.4), and to prepare brain samples for sulphamidase assays standard capillary depletion protocols were used.
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