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We acknowledge the electron microscopy facilities in the Department of Anatomy and Neurobiology at UC Irvine, and the Department of Pathology at Duke University, for assistance in preparing and imaging specimens.
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For time-lapse imaging, inflorescence apices were prepared and imaged as described [ 2].
A 72 hpf Platynereis larva (HT9-4) was prepared for serial sectioning and imaging as previously described (Conzelmann et al., 2013; Randel et al., 2014).
HmlΔ-nuclearDsRed; Lz-GAL4>EGFP/mCD8GFP early L3 male larvae (<12 hr after L3 ecdysis) were selected and prepared for imaging (see 'Materials and methods').
After fixation, cells were briefly permeabilized (5 min) with 0.5% Triton-X and prepared for imaging by washing in PBS, aspirating the washed solution, and adding a 1 ng/ml DAPI solution.
After fixation, cells were briefly permeabilized (5 min) with 0.5% Triton-X and prepared for imaging by washing in PBS, aspirating the washed solution, and adding a 1 ng/ml DAPI solution to stain DNA and a generic protein stain (CellMask, Invitrogen) to facilitate cell and nuclear image segmentation.
Samples were washed and prepared for imaging by freeze-drying.
Afterwards, slices were fixed and prepared for imaging as described above.
Porcine skin tissue was topically exposed to alumina, ceria, and silica NPs using a modified Franz diffusion chamber and histologically prepared for imaging.
Embryos were prepared for imaging as previously described (Wood and Jacinto, 2005) and imaged live on a Zeiss LSM510 confocal microscope using Zeiss fluar 40×/1.3 oil DIC, plan-apochromat 63×/1.4 oil and c-apochromat 63×/1.2 water immersion objectives at the Bath BioImaging Facility.
Fluorescent microscope samples were prepared on imaging slides, and 50 000 cells/well of each cell line were seeded into a 4 chambered imaging slide.
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