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Nested PCR is a recognized highly sensitive technology for HPV DNA detection and for preparing a template for DNA sequencing.
Similar(59)
We prepared a template file for A. thaliana microarray ATH1.
The TPM library prepares a command template for each TPM command in the runtime stack.
Fifty individuals of C. sinicus from the same population (the Yellow Sea) were pooled to prepare a DNA template for mitogenome sequencing.
For more details and a template for preparing your manuscript, see Science Robotics Information for Authors.
Similarly, L35 V/L74F was used as a template for preparing library DC2.
The furin cDNA was then used as a template for preparing the furin constructs.
In a first step, polystyrene sphere colloidal crystals were prepared in order to form a template for further reaction.
A copolymer containing 91 mol% N-isopropylacrylamide (NIPAM) units and 9 mol% N,N- dimethylaminopropyl)methacrylamide (MADAP) uN,N- dimethylaminopropylprepared using polyacrylate chains as a temethacrylamide positively charged MADAP units.
Briefly, cDNA prepared from HOZOT was used as a template for amplification, and PCR was performed with AccuPrime-Taq (Invitrogen, Carlsbad, CA) using the sets of specific primers (Table S1).
Oligonucleotides were prepared using M. tuberculosis DNA as a template for PCR, and sequencing was performed by the Bioresource Center at Cornell University.
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