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Nested PCR is a recognized highly sensitive technology for HPV DNA detection and for preparing a template for DNA sequencing.
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Following library preparation, Ion OneTouch system was used to prepare a template and sequencing was performed using an Ion 316 chip on an Ion PGM sequencer and results were analysed using Ion Ampliseq CHPv2 regions and hotspots, hg19 as reference in single sample analysis.
We prepared a template file for A. thaliana microarray ATH1.
A clone was grown in the presence of kanamycin, purified using a Qiagen maxi prep kit, and the resultant plasmid linearized with SpeI to prepare a template that made a 768 base antisense riboprobe that was used without hydrolysis.
The implant placement plans were then established, and the data were sent to a preparation center to prepare a surgical template.
The TPM library prepares a command template for each TPM command in the runtime stack.
We prepared a damaged template DNA produced with an alkylating agent (N-methyl-N-nitroso urea; MNU) to measure incorrect base-incorporation during DNA replication in microgravity.
Fifty individuals of C. sinicus from the same population (the Yellow Sea) were pooled to prepare a DNA template for mitogenome sequencing.
For 258 of the genes that scored as hits in the primary screen, we generated templates for preparing a minimally overlapping (0-11nt) validsRNAn dsRNA.
Even large-scale films can be easily prepared via a template synthesis.
The disulfide-crosslinked poly(γ-glutamic acid) hydrogels were prepared as a template hydrogel.
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