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The thin fibers were produced by preparing a solution of 0.1 mg/ml fibrinogen and 0.1 U/ml thrombin 1 hour in advance and then allowing it to react with the gel for 1.5 hours.
A dilution curve of the TCAD regimen was created by first preparing a solution of all three drugs at 100-fold the Cave of each drug (43 µg/mL amantadine, 130 µg/mL ribavirin, 30 µg/mL oseltamivir carboxylate), and then serially diluting this solution in half-log10 increments.
A dilution curve of TCAD regimen was created by first preparing a solution of all three drugs at 100-fold the Cave of each drug (43 µg/mL amantadine, 30 µg/mL oseltamivir carboxylate, and 130 µg/mL ribavirin), and then serially diluting this solution in 0.5-log10 0.5-log10s.
Each secretome sample was processed, by preparing a solution of 1 μg/ μL of proteins and 40 mM of ammonium hydrogen carbonate (Sigma-Aldrich, USA).
Procedures: Film formation was achieved by preparing a solution of PIM-Trip-TB (0.50 g) in chloroform (20 mL) which was poured into a 9 cm circular Teflon mould.
The deprotected N-terminus was acylated by preparing a solution of bromoacetic acid (90 μmol) and DIC (90 μmol) in DMF (500 μL) to activate the bromoacid (2 min, RT), transferring the activated bromoacid (500 μL) to the resin and incubating (15 min, 37 °C).
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Based on our results, we have prepared a solution design guide for preventing condensation and mold around built-in furniture.
The calculation for the amount of salt required to prepare a solution is given by Kabeel (2007).
Briefly, 6.62 mg of potassium persulphate, K2S2O8 was dissolved in 10 mL of distilled water to prepare a solution of 2.45 mM.
The preanalytical phase was to prepare a solution of ethyl esters of fatty acids (FAc) and diethylacetals of fatty aldehydes (FAl) of blood plasma samples by acidic ethanolysis.
To evaluate the formation of the AafA/fibronectin complex, we prepared a solution of 5 μg/mL of AafA- dsc protein and 10 μg/mL of purified fibronectin.
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