The quantitative questionnaire was prepared by referencing previously published literature [ 2, 10, 11]; but was contextualized into the local setting.
To complete the solvation free energy function, it was required to prepare a reference dataset with which the atomic parameters could be optimized.
We also prepared a reference sample (as-grown QDs) embedding as-grown InAs QDs formed without the partial capping and annealing processes instead of embedding the In-flushed QDs.
In order to evaluate the completeness of transcriptome assemblies, we prepared a reference gene set consisting of vertebrate one-to-one orthologs.
To prepare a reference database, 268614 LSU sequences were collected from NCBI (Methods), of which 120617, 95458 and 14540 were fungi, metazoan, and viridiplantae, respectively.
Normal tissue was obtained from benign breast tissue outside the tumour from five patients to prepare a reference pool for cDNA microarray analysis.
For laboratory use, the absorbance can be measured by using the reflected or transmitted intensity normalized by the reference intensity; however, this normalization may be difficult for in vivo measurements because it is not possible to prepare a reference tissue without the target chemical.
For each assay, we prepared a reference calibration curve as described previously, [ 31] which containing a nine-concentration titration series representing the erbB2 and β-actin genes at 10-fold dilutions from 1.0 ng/μl to 1.0 × 10-8 ng/μl (see Additional file 2: Supplemental Figure S1).
We prepared a reference construct by digesting the HSV-TK promoter, between the BamH1 site and Hind III sites, from the pRL-TK vector (Promega Corporation, Madison, WI, USA) that expresses Renilla luciferase, and cloning this fragment into the pGL4.18 [ luc2p/Neo] vector (Promega) that expresses Firefly luciferase.
To ensure your bender is set to the correct angle, you can prepare a reference tool of 2 flat pieces of metal whose ends are attached to a pivot.
