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The concentrations of thymol and carvacrol were determined using the calibration curves prepared with standards of thymol and carvacrol, and cumene as internal standard.
Quantification of the FAMEs was based on integration of individual fatty acid peaks in the chromatograms and quantified using a 5-point calibration curve (0.5 2 mg mL−1) prepared with standards containing 14 even carbon chain FAMEs (C8 C24, SIGMA cat #18918) and 5 odd carbon chain FAMEs (C13 C21, SIGMA cat #1896).
Similar(58)
The average chemical purity of α-cellulose laths from the five species was 94.5%, similar to the chemical purity of α-cellulose prepared with the standard Jayme-Wise method.
By contrast, a tall latte prepared with Starbucks's standard espresso blend offered the scorched, char-heavy flavors of beans roasted to death.
The system was calibrated with prepared standards.
Compound identifications were also confirmed by comparison with prepared standards of individual compounds.
The absolute configuration of the product was determined by high-performance liquid chromatography (HPLC) on a chiral stationary phase by using chemically prepared authentic standards with known dl- threo or l- threo configuration.
Sample blanks were similarly prepared but without skin samples and analysed with standards (+ 0 2 mg/L) prepared in 10% nitric acid.
Then add 40 ng of freshly prepared internal standards and continue with the protocol described in Section A. Note.
In all cases, add 40 ng of freshly prepared internal standards and continue with the protocol described in Section A. Defrost the sample on ice and in the dark but do not use a water bath (see comments in Section A).
The analytical evaluation of assays was performed with the prepared standards.
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