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Silica particles with diameters of 250 nm to 1.1 μm and low dispersities were prepared using a variation of the method developed by Stöber et al. [18].
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The implant bed was prepared using a sequence of drills.
Standards tubes were prepared using a solution of H2O2 50 mM.
Longitudinal sections of 5 μm were prepared using a microtome.
To account for variation between experiments, additional slides were prepared using an established lymphoblastoid cell line (Raji) and these were irradiated and analysed in parallel.
PCR amplification was prepared using an SYBR Green Real-time PCR MaSYBR Green Real-time
Using a variation of the approach above, we prepared DRMs from normal erythrocyte membranes by flotation from an alkaline 50% sucrose fraction, as described previously [18].
Chromatin was prepared with the Millipore/Upstate Chromatin Immunoprecipitation (ChIP) Assay Kit (Millipore) according to the instruction provided by the producer, using a variation of the protocol described at the Upstate website.
It said it used a variation of the Black-Scholes approach, the most widely-used method for valuing stock options.
A standard curve was prepared using serial dilutions of PGE2.
A standard curve was prepared using different concentrations of Trolox.
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