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In the present study, a PK-15 cell-adapted formalin-inactivated prototype vaccine candidate was prepared using a strain of PCV2 from China.
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The implant bed was prepared using a sequence of drills.
Standards tubes were prepared using a solution of H2O2 50 mM.
Furthermore, in a previous study of ENCEVAC® in Japanese children [ 12], a JE-VC was compared with a JE-MB prepared using a homologous JEV strain (Beijing-1).
Genomic DNA of H. pylori strains was prepared using a QIAamp DNA mini kit.
The rabbit antiserum specific to L. interrogans strain Lai was prepared using a modified procedure as previously described[ 18].
Plasmid DNA was prepared using a kit purchased from Qiagen after the propagations of the plasmid carrying strains for a period of time, normally overnight cultivation was applied.
Chemically competent XLI-blue strain Escherichia coli cells were prepared using a modified protocol from Inoue et al. [ 49].
Longitudinal sections of 5 μm were prepared using a microtome.
The IFA slides were prepared using the MARV strain of the index patient.
A phage lysate was prepared using the donor strain; for this, 200 μl of an overnight culture of the donor, a phage concentration of 5 × 10 PFU/ml, and 1 ml of LB broth (Bacto, Franklin Lakes, NJ) were mixed and incubated for 12 16 h at 37°C.
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