Sentence examples similar to prepared transformation from inspiring English sources

Furthermore, we prepared transformation-competent E. coli and S. cerevisiae ourselves and transformations were carried out as described previously with slight modifications (Hoffman and Winston, 1987; Tu et al., 2005; see materials and methods for details).

To prepare transformation complex, the DNA was diluted with Ham's F12 to a concentration of 2 μg plasmid DNA-Mix/100 μl Ham's F12. 100 μl diluted DNA/transfection were mixed with 7 μl FuGene HD in a sterile polystytrene tube without allowing contact with the walls of the plastic tube and mixed gently by vortexing.

Polystyrene (PSt /polytetrahydrofuran (PTHF) A2B2 miktoarm star copolymers were prepared by transformation of cationic ring-opening polymerization (CROP) to atom transfer radical polymerization (ATRP).

rBCG strain over-expressing α-crystallin of M. tuberculosis was prepared by transformation of BCG with a recombinant plasmid (pSD5.hsp65.acr) engineered to over-express α-crystallin under the transcriptional control of promoter of the hsp65 gene of M. leprae (Fig. 1A).

Once the OD reached 0.4-0.6 0.4-0.6lls were harvesthe and prepared for transformation.

P. pastoris GS115 cells were prepared for transformation according to the manufacturer's instructions (Invitrogen).

A large quantity of the commercial C3 plasmid was prepared with transformation of E. coli in advance.

Competent cells of E. coli DH5α and B. licheniformis WX-02 were prepared for transformation of constructed plasmids as described previously [ 23, 27].

Regular residential children's homes are prepared for transformation and their capacities freed for establishing small-scale homes for children with disability, and standards of practice in maternity wards aimed at discouraging institutionalization have been integrated into guiding regulations.

E. coli cells co-expressing CaM and recombinant FLAG BRI1 proteins were prepared by transformation of plasmids designed to express CaM proteins into E. coli BL21 DE3) pLysS cells (Novagen) harbouring a FLAG BRI1 expression plasmid.

For preliminary assays of enriched acrB libraries, the pre-culture was prepared by transformation of the mixture of plasmids into electrocompetent JA300A cells and growing the cells overnight in 10 mL LBA medium after 1 h of outgrowth.