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Polystyrene beads coupled covalently to specific antibodies (cytokines/chemokines) were prepared to react with an approximate amount of 20 μL of each tear sample (which contains an unknown amount of these molecules), or with a standard solution (having a known amount of molecules), at room temperature for 1 hour.
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Be prepared to react quickly with a held ball.
McClintock [ 37] addressed the presence and activity of mobile elements within the host as a chance for the genome to cope with challenges to which it is not prepared to react.
It will also need to be vigilant for signs that the dollar is being unduly pressured, and be prepared to react.
HOUSTON — As hopes dim for containing the oil spill in the Gulf of Mexico anytime soon, more people are asking why the industry was not better prepared to react.
Though President Bush told the nation this month that a single "Iraqi intelligence operative" could with one "small container" wreak havoc with chemical and biological weapons, we are largely defenseless against such an attack: "Police, firefighters and emergency medical personnel in most of the nation's cities and counties are no better prepared to react now than they were prior to September 11".
EPU was then produced by allowing the as-prepared polyurethane prepolymer to react with ACAT under suitable conditions.
The prepared monochlorotriazine was allowed to react with hydrazine and subsequently it was reacted with 2-methyl-4H-benzo[1,3]oxazin-4-one to obtain novel triazine-quinazolinone based hybrids (9a-f).
CS-A (450 mg), dissolved in 45 mL coupling buffer (0.1 M sodium bicarbonate buffer, pH 8.3, containing 0.5 M NaCl), was added to the prepared Sepharose (4500 mg) and allowed to react with agitation for 2.5 hours.
The mouse monoclonal antibody (mAb), NC-1, raised against the gp41 six/helix bundle from HIV-1 IIIB was prepared as described [ 43] and shown to react also with six-helix bundles from HIV-1 BaL [ 37].
The two-step method is that the PbI2 films were first prepared and subsequently reacted with MAI to form CH3NH3PbI3 films.
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