The prepared protein structure was used to generate a grid using the receptor grid generation utility of the Glide docking module of the Schrodinger suite [ 40, 41].
(5), where the spin is located at the oxygen atom of the terminal Al-Ot unit, there is no need to generate a "prepared state"; consequently, the reaction is barrier-free.
In contrast this England team have been meticulously and expensively prepared, flying business class to Australia three weeks ago to generate a mood of quiet confidence that they might be capable of springing a surprise.
For assay calibration, various amounts of recombinant human sPLA2 (prepared as described, Singer et al, 2002) were added to assay buffer to generate a standard curve.
To generate a more comprehensive data set, the final PCR products were prepared for Ion Torrent sequencing, following manufacturer's instructions, rather than subcloning into pCR4-TOPO.
The vaccine is designed to generate a self-replicating vector RNA (replicon) based on Sindbis virus [ 42] and was prepared as follows.
Wait for Complainterator to generate a message.
Be prepared to get a urine sample from your child.
We then use these prepared probabilities to generate false positives and false negatives in the experiments, thus introducing the same bias/systematic error.
