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GM conducted laboratory work and prepared the sequence alignments.
SM carried out the molecular genetic studies and prepared the sequence alignment.
CLC and CLW prepared the sequence data and submitted it to GenBank.
AB prepared the sequence data for analysis, performed the accuracy and speed tests and analyzed the results with ERMT.
OF and SM prepared the sequence alignments and ST and KO did the SNP validations and variant analysis.
LT compiled the information for the database, prepared the sequence alignments, helped to create the website front-end and drafted the manuscript.
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As an input control, we amplified control plasmid DNAs isolated from transfected cells in 10 independent PCR reactions (15 cycles, 2 ng plasmid DNA per reaction) and prepared the sequencing libraries as described above.
GH prepared the sequencing samples, participated in the bioinformatic analyses, conducted the genotyping, and evaluated the genotyping data.
PB performed data analysis, LJ prepared the sequencing libraries and MK helped with the annotation of sequences obtained through deep sequencing.
Therefore it is feasible to spend one hour or so preparing the sequence of configurations that will be used for testing a specific board.
To prepare the sequence template (nucleotides 75-638 of 5'NTR of HAV), PCR products were treated with ExoSAP-ITR (Affymetrix, Inc., Santa Clara, CA), and then sequenced using a BigDye(R) Terminator v3.1 Cycle Sequencing Kit (Life Technologies, Tokyo, Japan).
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CEO of Professional Science Editing for Scientists @ prosciediting.com