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Seven test samples were prepared spanning the concentration range of 103 106 ffu/mL.
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A truncated version of ZFD was also prepared, which spans residues 259 338, with the last 20 flexible residues removed.
High physical stability of the niosomes prepared from Span 40 and Span 60 was demonstrated due to negligible size change of vesicles during 6 months storage at 4 8°C.
Also, the niosomes prepared using Span 60 were larger in size than niosomes prepared using Span 40.
Since RBPs usually interact with 3′UTR of target mRNA and affect their expressions, we prepared biotinylated fragments spanning 3′UTR of Drp1 mRNA (3U1 and 3U2) and investigated interactions between hnRNP A1 and biotin-labeled 3U1/3U2 fragments by Western blotting.
Different niosomal formulations were prepared by Span and Tween mixed with cholesterol.
Primers used to prepare biotinylated transcripts spanning SASP mRNAs are listed below.
Confirmation that NF90 was capable of interacting with these 3'UTR segments was obtained by preparing biotinylated RNA spanning the 3'UTRs of these mRNAs (Methods).
As part of this study, we prepared luciferase reporter constructs spanning approximately 0.5 kb of genomic DNA upstream of each putative HAS transcription start site (TSS) [ 43, 44].
I also took into account the more recent verdicts from historians and viewers prepared for C-Span's "American Presidents" series in 1999.
Phase A: MRS phantoms will be prepared (expected life span 2 years) and scanned in 3 T MR scanners at the participating centres.
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