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The average baseline concentration of all the freshly prepared solutions of capsaicin prepared with Tween-80 was 83.9 ± 6.8% of predicted, with a range of 74.4 94.1%.
Stock solutions of ascorbic acid were prepared with doubly distilled water and only freshly prepared solutions were used.
While freshly prepared solutions with Tween-80 had consistently higher concentrations than those prepared without Tween-80 (83% vs. 69%), Tween-80 does not facilitate complete solubility.
Prepare solutions at least one step ahead and put these prepared solutions in water bath with sample vial.
The prepared solutions of puffball were filled into the wells.
Prepared solutions were stored at 4°C until use.
Only freshly prepared solutions of these substances were used.
All prepared solutions were kept at 4 °C until use.
Then the prepared solutions were stored overnight for complete hydration.
The proposed method was applied to the prepared solutions.
The prepared solutions were then vortexed for 1 min.
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