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All four solvents were prepared separately for extracting phenolics from leaves: methanol, ethanol, acetone and deionized water (ddH2O).
The inoculum was prepared separately for PSM and rhizobia using National Botanical Research Institute's Phosphate (Nautiyal 1999) and N-free medium, respectively, in Erlenmeyer flasks (250 ml) which were incubated for 48 h in orbital shaking incubator (Model OSI-503 LD; Firstek Scientific, Japan), at 28 ± 1 °C and 100 rev min−1.
Reference RNAs were prepared separately for the time course experiments and the steady state experiments.
An enriched microsatellite library was prepared separately for C. gunnari and N. rossii.
Other skeletal muscle was prepared separately for mitochondrial quantification using electron microscopy.
Total cell lysates of migrated cells and spheroids were prepared separately for protein expression.
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Shot by Hurley on Video 8 and edited on an iPhone, the video for "Reaching" follows a young couple over a long night out, beginning with the two parties preparing separately for the dalliance ahead.
The fish samples for microbiological analyses were prepared separately from the fish samples for physico-chemical analyses.
For each patient or healthy control, serum was prepared separately from PBMCs from the same blood draw.
Concentrations of 5 mg/ml of VOPcPhO and 5 mg/ml of PCBM solution were prepared separately in chloroform for a templating method of layer-by-layer assembly.
An aseptic rMnSOD solution was prepared separately in water for injection and sterilized by Millipore 0.22 μm filters; it was added to the gel formulation to give the required final concentration of 2.0 μg/mL.
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