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Starting with a microphotograph of a prepared section of the porous sample, the proposed algorithm, in a first step, covers the pore space of the digitized image with convex areas.
The prepared section was finally removed from the saw blade.
In a destructive method, only the SSD in the prepared section can be measured.
The prepared section or samples were photographed under a light microscope (Olympus SZ61, Tokyo, Japan).
As a control we used an IHC prepared section of the intestine infected with the original TgNmBr1 strain that was used to harvest merozoite mRNA.
Each prepared section was incubated with 10 μL of the probes, covered with a cover slip and placed in the StatSpin Thermo Brite hybridization system (Abbott Laboratories, Abbott Park, IL, USA) under the following conditions: denatured at 90°C for 10 min and hybridized at 47°C for 48 h.
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Standard paraffin preparations were prepared, sectioned at a thickness of 5 μm, and stained with hematoxylin and eosin.
Prepared sections were stained with HE staining, Masson staining, and toluidine blue staining.
Other projects prepared sections for eventual widening.
Osteocyte density was determined in similarly prepared sections.
Retina samples were prepared, sectioned and immunolabelled according to Tokuyasu [47].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com