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Freshly prepared medium was analyzed aside the samples as background control.
Viruses encoding Oct4, Klf4, c-Myc, and Sox2 were introduced into the cells twice, on Day 0 and on Day 1, and mES or mES-Vc (high glucose DMEM, NEAA, GlutaMAX, Leukemia Inhibitory Factor (PeproTech), 2-mercaptoethanol (Thermo Fisher), and 10% FBS without or with Vc (Sigma) was used on Day 2. The medium was replaced daily with freshly prepared medium.
After 15 days, the cells were harvested and introduced onto the above mentioned freshly prepared medium.
Cells maintained in prepared medium without adding tested samples were taken as a control.
Then, 1 mL of the freshly prepared medium, supplemented with 40 μg/mL neutral red (NR), was added to each well of both the cell culture plates.
The fifth course was the 7% Wagyu Beef Tenderloin, a perfectly prepared medium rare.
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Pipette 200 μL of the freshly prepared elution medium in the same sample preparation vial.
Quiescent TG1 cells cultured during 5 days at pH 6.5 were transferred to freshly prepared NS34 medium at pH 7.4.
Magnetic properties and performance characteristics of prepared disk medium with fundamental design idea have been discussed.
The reducing power was evaluated by measuring reduction potential of the prepared reaction medium.
Afterwards 200 μL of the freshly prepared elution medium was filled in the same vial.
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