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The prepared leaf extract was stored at room temperature to be used for biosynthesis of SNPs from silver nitrate.
The prepared leaf material was observed and electro-micrographed in a LEO EVO 40 XVP scanning electron microscope.
After duplicating the prepared leaf, its teeth are detached; typically, this is done with a straight line between the bounding sinuses of each tooth (see ref. [27] for exceptions).
We prepared leaf discs from the infiltrated areas of all three leaves from both plants at each time point, and pooled the samples for protein extraction and vIL-10 quantification by ELISA.
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The prepared neem leaf particles doped bioactive glasses nanoparticles are hereafter termed as NNBG.
Prepare the leaf powder.
Crude leaf DNA preparations were prepared according to [ 37].
Sections were taken above the first cauline leaf and prepared as described in [ 59].
The plant material was authenticated and prepared as root and leaf dry powder by Professor L J Liao.
The leaf explants, prepared as previously described [ 18], were incubated for either 24 or 48 h in the presence or absence of ethylene (10 μl/l).
Sweet orange leaf extracts were prepared as described previously for passion fruit leaf extracts [39].
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