To check if chloroplasts constitute a site of vitamin B6 accumulation in plant leaves, we prepared intact chloroplasts and we titrated vitamin B6 by HPLC (Additional File 4).
In order to circumvent these difficulties, we prepared intact cyanelles as described above and carefully solubilized outer membrane and periplasmic protein complexes according to the principles of blue-native gel electrophoresis [ 25, 26], a method not amenable to chloroplasts.
An exclusive method was developed to prepare intact tin whiskers as transmission electron microscope specimens, and with this technique in situ observation of tin whisker growth from RESn3 (RE = Nd, La, Ce) film specimen was first achieved.
Taken together, our findings outline a successful strategy to prepare intact pine pollen SECs and demonstrate for the first time that SECs can be prepared from multi-compartmental pollen capsules, opening the door to streamlined processing approaches to utilize pine pollen microcapsules in industrial applications.
Using several commercially available cell-wall digesting enzymes (such as β-glucanase, Driselase, and lyticase), we were unable to prepare intact CBS999.97 and QM6a chromosomes for clamped homogeneous electric field (CHEF) gel electrophoresis.
Thin sections of the soils were prepared from intact cores of the A horizon.
CMP was calculated via image analysis of resin-impregnated soil blocks prepared from intact soil specimens.
Total RNA samples from day8 wild type and VRK1-deficient mice (n = 4, each) were prepared from intact testicular cells using Trizol (Invitrogen, Carlsbad, CA) and then reverse transcribed using the ImProm-II Reverse Transcription System (Promega).
To test this hypothesis, reactions were prepared containing intact virions or detergent-lysed virions either with or without PGF, and they were subjected to ultracentrifugation after incubation at 37°C for 18 h (mimicking conditions used in the ERT assay).
Counter-intuitively, this implies that newly prepared, highly intact DNAs will be most vulnerable to TUF induced problems, whereas older and/or more degraded samples will be less affected.
While these objectives have been achieved individually (FACS based on intracellular antigens, high quality RNA prepared from intact flow sorted cells), to our knowledge they have not been combined in a carefully explored, robust protocol.
