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The recovery samples were prepared in triplicate to determine the reproducibility of the methods.
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One sample was prepared from each line and analyzed in triplicate to determine the concentrations of NA and DMA.
These were amplified by Mycoplasma FRET-PCR in triplicate to determine the detection limit of the PCR.
Cultures were sampled every 24 h over a 96 h period in triplicate to determine optimal conditions for bioreactor culture.
Each experiment was run in triplicate to determine the means and S.Ds.
Each dilution series was analysed in triplicate to determine relative sensitivity.
Each experiment was run in triplicate to determine means and S.D.s.
Samples were prepared in triplicate to facilitate statistical analysis.
All samples were prepared in triplicate to a final volume of 5 mL.
Blanks, standards and samples were prepared in triplicate to measure ASC.
After laser capture microdissection, direct quantitative real-time reverse transcription-polymerase chain reaction assays were performed in triplicates to determine HIF1a, PDGFA, VEGF, and bFGF gene expression levels.
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