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The samples were prepared as described above RNA samples from untreated and immune-challenged animals were prepared in each case from five late larvae (L2) and five adult minor workers (W2) of six different ant colonies (C90, C96, C152, C79, C264, C132) 12 h post infection.
The ICP-AES instrument was calibrated using a 6-point multi-element standard prepared in each solution.
Twenty five extracted third molars were used in the study; two box-like Class II cavities were prepared in each tooth (mesial and distal surface).
Films with 20, 40, 60, 80, and 100 bilayers were prepared in each growing configuration in order to study the effects of the construction parameters.
Because authentic standards such as NBS-19 are not largely available, the reference materials routinely used are prepared in each laboratory or community (e.g., Tayasu et al. 2011).
Depending on how the factors of safety are defined, the stability numbers computed using the two definitions may be different, and so the design charts prepared in each approach should be assessed with caution.
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Two master mixes were prepared in which each of the allele-specific primers were added.
To test MID-adaptors 1 12, 12 libraries of C. tentans DNA were prepared in parallel, each library with a different MID-adaptor.
Mixtures of SssI-treated DNA: untreated DNA (100%, 50%, 10%, 1%, 0.1% 0.01%) were prepared in duplicate (each containing 1.5 μg of template DNA).
They don't rehearse and nothing is prepared in advance, so each gig, each set is different from the last one.
The samples were prepared in triplicate, and each cell preparation was analyzed twice.
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