Sentence examples for prepared in a volume of from inspiring English sources

Briefly, 10,000 cells were prepared as single cell suspensions in 0.35% agar prepared in a volume of 1 ml of culture media, and then seeded onto 1 ml of hard agar (1% agar prepared in culture media) in 12-well plate.

Reactions were prepared in a volume of 10 µl using GoTaq Hot Start Green Master Mix (Promega).

Shortly, peptide dilutions were prepared in a volume of 1 ml in twofold dilution steps, in a concentration range from 0.75 to 96 μg/ml in 0.37 % BHI.

PCR reactions were prepared in a volume of 15 μL containing 1x PCR buffer, 1.5 mM MgCl2, 200 μM dNTPs, 200 nM of each forward and reverse primers, 0.02 U of GoTaq DNA polymerase and 1 μL of DNA template.

PCR reactions were prepared in a volume of 12.5 μl containing 10× Taq polymerase buffer (500 mM KCl, 100 mM Tris- HCI pH 8.5, and 1 mg/ml gelatin), 1.0 mM MgCl2, 0.5 mM dNTPs, 5 pmol of each primer, 0.25 U Taq polymerase, and 25 ng of template DNA.

In order to make fructose ingestion by F2 comparable to that of FG group, during each trimester of gentamicin treatment period, fructose solution containing the average amount of fructose ingested by FG group was prepared in a volume of water corresponding to the amount of water taken by F group during the correspondent period.

A solution of Microfil was prepared in a volume ratio of 4 5 of Microfil:diluent with 5% curing agent.

Reactions were prepared in a total volume of 20 μl containing: 2 μl of template, 10 μl of 2×SYBR Premix, 0.4 μl of each specific primer to a final concentration of 200 nM.

Reactions were prepared in a total volume of 20 μl containing: 4 μl of template, 2 μl of each amplification primer (optimized concentration in table 2), 10 μl of 2× FastStart SYBR Green Master Roche Applied Sciencee) and 2 μl of fluorescein as normalization dye.

Reactions were prepared in a total volume of 8 μl containing: 1 μl of template, 0.8 μl of each amplification primer (2 μM), 4 μl of SYBR® Green JumpStart™ Taq Ready Mix™ (Sigma-Aldrich St . Louis MO, USA) and 1.4 μl of sterile dd water.

qRT-PCR reaction solution was prepared in a total volume of 20 μL, containing 2 μL of the cDNA, 0.2 mM of each primer, and 10 μL of 2 × SYBR green PCR master mix (Takara Co. Ltd., http://www.takarabiomed.com.cn/).