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The solutions of different concentrations of Bi III) ions ranging from 0.001 to 1 ppm were prepared in a buffer solution of pH 4.
The hENT1 protein was prepared in a buffer containing 0.02% DDM, 30 mmol/L HEPES pH 7.4, and 150 mmol/L NaCl.
Three hundred microliters of 0.8 mM 2H/13C/15N-Ube2g1 was prepared in a buffer (pH 7.0, 50 mM HEPES, 100 mM NaCl, 5 mM DTT, and 5%% D2O) and then was transferred into a Shigemi tube.
Aqueous solutions of Cd (II) and Ni (II) ions for the batchwise experiments were prepared in a buffer solution (0.1 M HNO3 and 0.1 M HEPES [ N-[2-hydroxy ethyl] piperazine-N′-N-[ N-[2-hydroxynic acid])], pH 3.0.
These samples were prepared in a buffer solution at pH 7.2.
Analysts were prepared in a buffer containing 10 mM HEPES (pH 7.4), 150 mM NaCl, 0,005% v/v Surfactant P20.
Similar(26)
CD was performed on a Jasco J-810 spectropolarimeter with purified myoc-OLF (8 10 µM) prepared in Buffer A, Buffer A adjusted to pH 5.8, as well as in 10 mM sodium acetate/acetic acid and 200 mM NaCl, pH 4.6.
Generally, liposome is prepared in a neutral buffer solution and thus the loaded drug molecules are also in a neutral environment after incorporation into liposome.
The solutions of surfactants were prepared in a saline buffer with similar pH and saline composition as the OptiMEM media that was used in cell incubation.
Supernatants and lysates were harvested and prepared in a sample buffer containing 1% β-mercaptoethanol.
Whole-cell lysates were prepared in a lysis buffer containing NP40 supplemented with a protease inhibitor cocktail (Roche).
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