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Each PCR plate included three no-template control samples and a standard curve based on 0-2ng bisulphite converted fully methylated human DNA (Merck-Millipore, MA, United States) prepared in a background of nuclease-free water (Promega, WI, United States).
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A dilution series of BRAF V600E mutant DNA (HTB-38D) from a HT-29 cell line (ATCC) was prepared in a high, constant background (5 000 copies/μL) of wildtype DNA (NA19205, Coriell).
A reference curve was prepared by amplifying serial dilutions of LV-encoding plasmid in a background of genomic DNA isolated from untransduced murine colon.
Briefly, a standard curve was prepared using a 1∶1∶1 ratio of genomic DNA from chronically HIV-1 infected cell lines ACH-2 [39], 8E5/LAV [40] and OM-10.1 [41] in a background of HeLa genomic DNA (data not shown).
Be prepared in case of an emergency.
For preparing the suspensions, a background solution of 1 mM KNO3 was used.
After weeks of preparing in the background, they suddenly become the most important person on campus.
Many effects require the actors to perform against a background of previously prepared film.
We prepared a monolayer presenting maleimide groups at a density of 10% against a background of tri ethylene glycol) groups.
Sand paintings are stylized, symbolic pictures prepared by trickling small quantities of crushed, coloured sandstone, charcoal, pollen, or other dry materials in white, blue, yellow, black, and red hues on a background of clean, smoothed sand.
The peptides were all prepared in the N-acetylated form and were immobilized by applying solutions to a monolayer presenting maleimide groups at a density of 1% against a background of tri ethylene glycol) groups.
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