Sentence examples for prepared from the pool from inspiring English sources

The reads generated by the sequencing of DNA prepared from the pool in a single Illumina flow cell were counted and assigned to the barcodes expected to be in the pool.

The Fc of the IgG1 was prepared from the pool by a common procedure including papain cleavage, purification with MabSelect, and Superose12 (Amersham Biosciences) gel filtration chromatography to analyze the Fc oligosaccharide structure.

For cytoplasmic screening, plasmid was prepared from the pool of bacteria of the 2nd or 3rd selection round, digested with NcoI and NotI enzymes, and the 750 bp band was cloned in NcoI- NotI digested and dephosphorylated plasmid pET23NN.

Data was obtained from myobundles during 4 weeks in culture prepared from the same pool of MHCK7-GCaMP6 transduced myogenic precursors.

For each of the three phenotypes, two distinct cDNA libraries were prepared from the same pool of total mRNA and sent at the National Genotyping Center (Genoscope, France) and sequenced individually on the Illumina Genome Analyser II system (GAII) to assess technical variations.

These primers (sense, 5′-GGTTTATCCTTAGAAAGGAGGT-3′, and antisense, 5′-GAGCATTCGGTTGGGCACTCTAG-3′) were used to amplify G. adiacens from DNA prepared from the bacterial pools isolated from maternal cervix and, as control, from DNA prepared from the infant's blood culture isolate.

We next looked at tetramer binding characteristics of Th1, Th2 and Th17 polarized, non-transgenic T cell lines cultured for eight days in polarizing medium and prepared from the same initial pool of primed LNC, using H2-Ag7 tetramers loaded with either PLP56 to 70 or an irrelevant H2-Ag7-binding peptide (CLIP103 to 117, PVSKMRMATPLLMQA).

Sequencing libraries were prepared from these pools via essentially the same approach used for generating labelled shRNA barcodes for the GMAP array, followed by an additional amplification step to incorporate adapter regions (see Materials and Methods).

A P labelled probe was prepared from the inserts of the pool of these selected clones using PCR and primers corresponding to either end of the multicloning site of the vector.

When Cy3- and Cy5-labeled cDNA was prepared from the same uninfected RNA pool and simultaneously hybridized on the array, none of the probes that hybridized differentially in the 12- and 18-h comparisons hybridized differentially (results not shown).

Total RNA was prepared from the B lymphocytes and pooled together.