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Third, to analyze the within-platform variations, Cy3- and Cy5-labeled cDNAs were prepared from a single preparation of total RNA from unstressed cells, mixed together and hybridized to a microarray.
Materials were all prepared from a single batch of medical grade GUR 1050 resin (Ticona, Bayport, TX).
The 6.6 20.0 mg L−1 P2O5 standards were in-line prepared from a single stock solution.
Overnight Mueller Hinton (MH) broth (Oxoid, England) culture was prepared from a single colony and its turbidity was adjusted in accordance with the 0.5 McFarland standard solutions.
The bacterial suspensions were prepared from a single colony, using 2 ml of 0.45% sterile saline solution to the equivalent of a 0.5 McFarland turbidity standard.
If the constituents of composites could be solubilized and chemically bonded than the formed insoluble composite could be prepared from a single type of polysaccharide.
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Total RNA was prepared from a whole single adult of A. lumbricoides and A. suum respectively, using Trizol reagent (Invitrogen) according to the manufacturer's protocol.
Cobalt-doped titanium dioxide nanobelts which showed room temperature ferromagnetism were prepared from a ferromagnetic rutile TiO2 single crystal.
PrPres features are assessed by analyzing a single homogenate prepared from a brain fragment from the animal.
The PSiO2 surfaces are prepared from a highly doped p-type single-crystal Si wafer, polished on the <100 > face using an anodic electrochemical etch.
The conidiogenous cells from the initial slide culture, held 7 days and prepared from a PFA block, consisted predominately of single, long, tapering phialides, somewhat atypical for the species.
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