Sentence examples for prepared for staining from inspiring English sources

Slides were prepared for staining as described previously85 and incubated with 1 μg/ml biotinylated lectins SNA, PNA, and MAL II (Vector, Burlingame, CA) and then stained following the manufacturer's instructions.

After 12 h, they were then washed with PBS and prepared for staining using a fixative solution for 10 min at room temperature, and the nuclei were stained with 4', 6-diamidino-2-phenylindole (DAPI).

Sections were placed on Superfrost Plus slides (Fisher Scientific, Pittsburgh, PA) and prepared for staining as described [6].

Serial sections of the eyes were cut at 10 µm thickness on a cryostat (CM1850; Leica, Heidelberger, Nussloch, Germany) at −20 C, and prepared for staining.

Upon each passage of ISE6 cells exposed to booklice homogenates, a portion of the cells were prepared for staining using a Cytospin centrifuge (Wescor) and rickettsial infection was assessed by traditional PCR [15] and/or Diff-Quik (Dade Behring) staining, according to the manufacturer's protocol.

After that, cells were harvested and prepared for staining protocols.

To prepare for staining, the slices were prewashed in PBS (0.01 M, pH 7.4) three times for 5 min each, then sequentially treated with 0.2% Triton X-100 in PBS for 5 min and with 0.3% H2O2 in PBS for 10 min, and washed in PBS three times for 5 min each, all at room temperature.

Paraffin-imbedded tissues were prepared for IHC staining.

If isolates were oxidase and catalase, smears were prepared for Gram staining.

Consecutive sections (5 μm) from paraffin-embedded liver were prepared for HE staining.

Tissue sections of 4 μm thickness were prepared for all staining.