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Total RNA was isolated from control and MERRF muscle, and tRNA libraries were prepared for sequencing.
Microbeads were counted, and amplified by PCR, and the 3′ end of the cDNA was prepared for sequencing.
The DNA isolated from DN422 and KY131 and the two DNA pools were prepared for sequencing.
LacZ genes of selected mutant plasmids were prepared for sequencing.
Once mutants were identified, individual samples were prepared for sequencing.
At least five clones from each PCR product were then prepared for sequencing.
The cells can then be lysed, bisulfite treated, and prepared for sequencing.
The contents of each microfluidic well were amplified individually and prepared for sequencing.
Plasmid DNA of selected clones was extracted and prepared for sequencing.
An equimolar pool of the 27 libraries was prepared for sequencing.
Total DNA was extracted using phenol-chloroform and prepared for sequencing as previously described [ 51].
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