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The PCR fragments were prepared for primer extension by incubating a mixture containing 7.5 μl PCR product, 0.5 U shrimp alkaline phosphatase (SAP) (TaKaRa, Otsu, Japan) and 1 U exonuclease I (TaKaRa) in a final volume of 10 μl at 37°C for 40 min, which was followed by inactivation of the enzymes at 80°C for 20 min.
In the behavioral experiment, 3 different velocities of visual hand motions were prepared for each flexion (CONG) and extension (INCONG) hand movement.
What is necessary for extension?
By extension, ECM prepared from decellularized cardiac tissue could be isolated, prepared into a powder, and fabricated into scaffolds as injectable materials or prepared for cell seeding.
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